Experimental design

A well-designed experiment is more likely to give reproducible, biologically-relevant results and next generation sequencing studies are no exception. NGS studies are time and resource consuming. It is well-worth giving enough time for designing experiments and avoid regrets later, especially if this is your first NGS project.

We help with designing your experiments; starting from how many samples, quality control of samples, and which technique or kit to use. We discuss the pipeline for data analysis post-sequencing. Prior to commencing a project, we go through the entire analysis and discuss various options appropriate for the biological question you are addressing.

Feel free to contact us if you would like to discuss your experimental design with us before starting your project. We do not charge for inputs in experimental design!

Data exchange

NGS typically generates several gigabytes of data. Once you have obtained the raw data, you can either send it to us for analysis by shipping an external hard drive or uploading the data to a secure website that we provide.

Data analysis

We analyse data using dedicated cloud-based server. Our section on What we offer covers the range of analysis tools that can be applied to probe your NGS data. Each experiment has a different requirement and as such each presents a unique challenge. Depending on the amount of data to be analysed and the extent of analysis, our analysis can take a few days to several weeks.

Results of analysis

Once the results are available, we discuss the results with you. After an in-depth discussion of analysis results with you, we can identify and perform additional downstream analysis as required. For example, this can involve analysis to address a specific question or generating publication-ready figures. Depending on the extent of the work, this will take an additional few days or few weeks. Once the analysis is complete, we can either upload the results at a secure site for you to download or send it to you on an external hard drive. We store your original data and results of analysis for three months (or longer, upon request) after the project is completed.

Cost

Each project is different and has unique requirements and challenges. Depending on the extent of analysis and the number of samples to be analysed, the cost can range from a few thousand to a few lakh rupees or a few hundred to a few thousand dollars. We will always provide you with a quote or a cost estimate before we start the analysis steps.